phi29 DNA Polymerase is a highly processive recombinant polymerase with exceptional strand displacement activity, which allows for highly efficient isothermal DNA amplification. The enzyme is capable of up to 70 thousands base insertions per binding event. The polymerase also possesses a 3’to 5’ proofreading exonuclease activity acting preferentially on ssDNA or RNA. Therefore 3′-modified primers are recommended.

Features

• Recombinant polymerase derived from the Bacillus subtilis phage phi29 and over-expressed in E. coli.
• Extremely processive polymerase (up to 70 kb) with very strong strand displacement activity, which allows for highly efficient isothermal DNA amplification.
• Extremely high yields of amplified DNA can be obtained even from minute amounts of template.
• High-Fidelity polymerase – possesses a 3’-5’ exonuclease (proofreading) activity acting preferentially on ssDNA or RNA.

Applications

• Rolling Circle Amplification (RCA).
• In situ genotyping with padlock probes.
• Amplification of DNA for SNP and STR detection.
• Unbiased amplification of whole genome.
• DNA template preparation for sequencing.
• RNA-primed DNA amplification.
• Multiple displacement amplification (MDA).
• Cell-free amplification of DNA from single cells.